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Анотація
In this paper, an optimal complex is selected of enzyme preparations for hydrolysis of the components of grain raw materials during fermentation of high concentration wort. When selecting enzyme systems, their effect on the technical and chemical parameters of the fermented wash during the fermentation of wort is investigated. For the research, maize grain with a starch content of 69.0 % was used. Fermentation was carried out with 18–30% of dry matters (DM) in the wort, using the osmophilic yeast strain Saccharomyces cerevisiae DO-16.The recommended concentration of the enzyme preparation Amylex 4 T (the source of the α-amylase enzyme) – 0.4–0.6 units of α-amylase ability/g of starch – is optimal for the concentration 18–27% of DS in the wort. For 30 % of DS, it is practical to use 0.6 units of α-amylase ability/g of starch. With the use of the enzyme preparation Diazyme TGA (the source of the enzyme glucoamylase), the value is 7.5 units of glucoamylase ability/g of starch, alcohol accumulation in fermented washes was 10.51, 13.35, 15.78% vol., according to the wort concentrations 18, 27, 30 %, respectively. It has been established that with the application of the cytolytic enzyme Laminex 750, the concentrations of dissolved carbohydrates and non-dissolved starch have a tendency to decrease.
In the samples where the proteolytic enzyme preparation Alphalase AFP was added at a concentration of 0.05 units of proteolytic ability/g of raw materials, there was an increase in the accumulation of yeast cells by 6.5% compared with the reference sample. The recommended concentration of Deltazyme VR XL (the source of β-glucanase and xylanase) is 0.05 units β-glucose/g of raw materials. The addition of a cytolytic and proteolytic enzyme preparation in combination with β-glucanase and xylanase contributed to an increase in the accumulation of ethanol in the washes by 1.7 % compared with the reference sample, and to an almost 33 % decrease in the concentration of dissolved carbohydrates and non-dissolved starch. On the basis of experimental studies, it has been found that using a complex of enzyme preparations – amylolytic (Amylex 4T), saccharifying (Diazyme TGA), proteolytic (Alphalase AFP), cytolytic (Laminex 750), and complex AF β-glucanase and xylanase (Deltazyme VR XL), in various combinations of their concentrations, – contributed to the intensification of the fermentation process of the wort and increased accumulation of the target product, ethanol, by 0.8–1.4 %, depending on the wort concentration. The highest amount of ethanol accumulated at the maximum dosage of additional enzyme preparations.
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Посилання
2. Kovalchuk S, Shiyan P, Mudrak T, Kuts A, Kyrylenko R. Investigation of the Effect of Nanoparticles of Metals on Fermentation of a High-Concentration Serum. Eureka: Life Sciences. 2017; 6(12):51-56. doi: 10.21303 / 2504-5695.2017.00512
3. Saha BC. et al. Pilot scale conversion of wheat straw to ethanol through simultaneous saccharification and fermentation. Bioresour Technol. 2015;175:17–22
4. Canilha L, et al. Bioconversion of sugarcane biomass into ethanol: an overview about composition, pretreatment methods, detoxification of hydrolysates, enzymatic saccharification, and ethanol fermentation. //J Biomed Biotechnol. 2012;15.doi: 10.1155/2012/989572.
5. de Souza CJ, et al. The influence of presaccharification, fermentation temperature and yeast strain on ethanol production from sugarcane bagasse.//Bioresour Technol. 2012; 109:63–69. doi: 10.1016/j.biortech.2012.01.024.
6. Moretti MMS, et al Effect of pretreatment and enzymatic hydrolysis on the physical-chemical composition and morphologic structure of sugarcane bagasse and sugarcane straw.//Bioresour Technol. 2016;219:773–777. doi: 10.1016/j.biortech.2016.08.075.
7. Binod P, et al.Bioethanol production from rice straw: An overview.//Bioresour Technol. 2010;101:4767–4774. doi: 10.1016/j.biortech.2009.10.079
8. Zhao X, et al.Enzymatic hydrolysis and simultaneous saccharification and fermentation of alkali peracetic acid-pretreated sugarcane bagasse for ethanol and 2,3-butanediol production. //Enzyme Microb Technol. 2011; 49(4): 413–419. doi: 10.1016/j.enzmictec.2011.07.003.
9. Irfan M, et al.Ethanol production from agricultural wastes using Saccharomyces cerevisiae.//Braz J Microbiol. 2014;2:457–465 doi.org/10.1590/S1517-83822014000200012.
10. Mielenz J.R.Ethanol production from biomass: technology and commercialization status.//Curr Opin Microbiol. 2001;3:324-329.
11. Geddes C.C., Nieves I.U., Ingram L.O. Advances in ethanol production. //Curr Opin Biotechnol. 2011; 22: Р. 312–319. doi: 10.1016/j.copbio.2011.04.012.
12. Aita G.A., Salvi D.A., Walker M.S..Enzyme hydrolysis and ethanol fermentation of dilute ammonia pretreated energy cane. //Bioresour Tech-nol. 2011; 6: 4444–4448.doi: 10.1016/j.biortech.2010.12.095.
13. Biragova NF, Birogova SR,Gotsunova NM, Eliauri RR Vliyanie fermentnyih preparatov na kachestvo potsessa osaharivaniya krohmalsoderzhaschego syirya. Proizvodstvo spirta i likerovodochnyih izdeliy. 2010;1:32-33.
14. Rosolova RO, Melnyk SR, Marynchenko VO. Osoblyvosti otsukriuvannia krokhmalevmisnoi syrovyny z vykorystanniam kontsentrovanykh fermentnykh preparativ// Kharchova i pererobna promyslovist. 2003; 1:18–19
15. Martins LH, Rabelo S.C, da Costa A.C. Effects of the pretreatment method on high solids enzymatic hydrolysis and ethanol fermentation of the cellulosic fraction of sugarcane bagasse //Bioresour Technol. 2015. Bioresour Technol. 2015;191: 312-321. doi: 10.1016/j.biortech.2015.05.024
16. Protsan N, Nykytiuk O, Oliinychuk S., Tkachenko L. Vplyv dopomizhnykh rechovyn ta fermentnykh prepa-rativ na hidroliz krokhmaliu zhyta // Kharchova i pererobna promyslovist.2010; 4:18–19
17. Rimareva LV, Overchenko LV, Polyakov VA. Rol proteaz v spirtovom brozhenii// Mikrobnyie bioka-talizatoryi dlya pererabatuyuschih otrasley APK: sb. nauch. tr. / VNIIPBT.M. 2006.M.127–137.
18. Palyanitsya L.Ya ProteolItichnI fermentnI preparati u virobnitstvI spirtu z krohmalevmIsnoYi sirovini-VIsn. Nats. un–tu "LvIv. polItehnIka". 2008;609:141–144.
19. Poligalina G. V. Tehno-himicheskiy kontrol proizvodstva alkogolnyih ta likero-vodochnyih izdeliya. G.V. Poligalina. Moskva : Kolos. 1999; 334 .