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Анотація
The advancement of scientific research in the development of novel functional food and feed products has led to a significant expansion of their range. Selecting effective ingredients for such products and determining the impact of subsequent processing technologies require assessing their level of functionality, which involves substantial investments of time and material resources. Therefore, the application of methods for evaluating the potential prebiotic properties of food and feed raw materials, as well as ready-to-consume products, is of great interest to both research and industrial laboratories. In 2003, R. Palframan, G.R. Gibson, and R.A. Rastall proposed a method for determining the prebiotic index (PI). This method involves calculating the ratios of cultivated bacteria of the genera Lactobacillus and Bifidobacterium, as well as bacteroides of the family Bacteroidaceae and bacteria of the genus Clostridium, grown on the same nutrient medium, to the total number of microorganisms grown on that same medium. This paper attempts to improve the aforementioned method for determining the prebiotic index by separately calculating the ratios of cultivated bacteria of the genera Lactobacillus and Bifidobacterium to the bacteria of the same genera grown on identical control nutrient media. Evaluating prebiotic indexes separately allows for a clearer differentiation of the raw material's functional effects compared to the integral prebiotic index, which averages out prebiotic characteristics, thereby complicating the targeted design of products for specific dysbiotic conditions and the needs of human and animal organisms. The determination of the prebiotic index based on the growth of Bifidobacterium and Lactobacillus bacteria upon adding samples of common vegetables to control nutrient media confirmed high selectivity regarding the growth stimulation of individual microbial genera. None of the tested products simultaneously exhibited maximum prebiotic index values for both lacto- and bifidobacteria, which experimentally confirms the validity of the developed approach for the separate calculation of indexes (BIlac. and BIbif.).
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Посилання
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