Food Science and Technology

ISSN-print: 2073-8684
ISSN-online: 2409-7004
ISO: 26324:2012



Vasfiye Hazal Özyur
Ayşegül Erdoğan
Zeliha Zeliha Demirel
Meltem Conk Dalay
Semih Ötleş


Recently, microalgae have become important in their health, and cosmetic applications since they are viewed as new sources of carotenoids. Fucoxanthin is also a type of carotenoid. The anti-diabetic, anti-obesity, anti-cancer, and antioxidant properties of fucoxanthin have been widely reported. Since these valuable properties, they also represent a valuable resource of nutraceuticals for functional food applications. This study aims to determine the amount of fucoxanthin, gallic acid, and rutin in Nitzschia thermalis obtained from the Ege University Microalgae Culture Collection. The extraction parameters have been optimized using response surface methodology. The extraction temperature (25, 35, and 45°C), the extraction time (10, 20, and 30 min) and the biomass/solvent ratio (0.005, 0.001, and 0.015 g ml-1) have been assessed as response variables in the Box – Behnken design. The amount of fucoxanthin was determined by the C30 column at 450 nm, while both the amount of gallic acid and rutin were separated in the C18 column at 275 nm by HPLC-DAD. In the present study, the optimum extraction conditions providing the maximum amount of fucoxantin, gallic acid, and rutin were selected by applying the “desirability” function approach in response surface methodology. Finally, the temperature has been determined to be 27.30°C, the extraction time 10 minutes, and the biomass ratio 0.05 g ml-1. Under these conditions, the optimum fucoxanthin level has been determined as 5.8702 mg g-1, the gallic acid level as 0.0140 mg g-1, and the rutin level as 0.0496 mg g-1. The findings are in good agreement with international published values for fucoxanthin content. In addition, response surface methodology was shown to be an effective technique for optimising extraction conditions for maximum fucoxanthin yield. In conclusion, these findings may be applied in the development of extraction methodologies for value added microalgea products as well as can serve as a reference for the extraction of fucoxanthin having high gallic acid and rutin from other brown microalgae, and therefore it could potentially be applied in both pharmaceutical and food industries.

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Як цитувати
Hazal Özyur, V., Erdoğan, A., Zeliha Demirel, Z., Conk Dalay, M., & Ötleş, S. (2019). OPTIMIZATION OF EXTRACTION PARAMETERS FOR FUCOXANTHIN, GALLIC ACID AND RUTIN FROM NITZSCHIA THERMALIS. Food Science and Technology, 13(1).
Хімія харчових продуктів і матеріалів. Нові види сировини


1. Jiang Y, Nunez M, Laverty KS, Quigg A. Coupled effect of silicate and nickel on the growth and lipid production in the diatom Nitzschia perspicua. 2014;
2. Shannon E, Abu-Ghannam N. Optimisation of fucoxanthin extraction from Irish seaweeds by response surface methodology. J Appl Phycol. 2017;29(2):1027-36.
3. Nakazawa Y, Sashima T, Hosokawa M, Miyashita K. Comparative evaluation of growth inhibitory effect of stereoisomers of fucoxanthin in human cancer cell lines. J Funct Foods. 2009;1(1):88-97.
4. Jaswir I, Noviendri D, Salleh HM, Miyashita K. Fucoxanthin Extractions of Brown Seaweeds and Analysis of Their Lipid Fraction in Methanol. Food Sci Technol Res. 2012;18(2):251-7.
5. Beppu F, Hosokawa M, Niwano Y, Miyashita K. Effects of dietary fucoxanthin on cholesterol metabolism in diabetic / obese KK- A y mice. 2012;1-8.
6. Das SK, Hashimoto T, Shimizu K, Yoshida T, Sakai T, Sowa Y, et al. Fucoxanthin induces cell cycle arrest at G0/G1phase in human colon carcinoma cells through up-regulation of p21WAF1/Cip1. Biochim Biophys Acta - Gen Subj. 2005;1726(3):328-35.
7. Namitha KK, Negi PS. Chemistry and biotechnology of carotenoids. Crit Rev Food Sci Nutr. 2010;50(8):1040-8398.
8. Kotake-Nara E, Sugawara T, Nagao A. Antiproliferative effect of neoxanthin and fucoxanthin on cultured cells. Fish Sci. 2005;71(2):459-61.
9. Peng J, Yuan JP, Wu CF, Wang JH. Fucoxanthin, a marine carotenoid present in brown seaweeds and diatoms: Metabolism and bioactivities relevant to human health. Mar Drugs. 2011;9(10):1806-28.
10. Hosokawa M, Kudo M, Maeda H, Kohno H, Tanaka T, Miyashita K. Fucoxanthin induces apoptosis and enhances the antiproliferative effect of the PPARγ ligand, troglitazone, on colon cancer cells. Biochim Biophys Acta - Gen Subj. 2004;1675(1-3):113-9.
11. Wang S, Verma SK, Hakeem Said I, Thomsen L, Ullrich MS, Kuhnert N. Changes in the fucoxanthin production and protein profiles in Cylindrotheca closterium in response to blue light-emitting diode light. Microb Cell Fact. 2018;17(1):1-13.
12. Poojary MM, Barba FJ, Aliakbarian B, Donsì F, Pataro G, Dias DA, et al. Innovative alternative technologies to extract carotenoids from microalgae and seaweeds. Mar Drugs. 2016;14(11):1-34.
13. Kotake-Nara E, Asai A, Nagao A. Neoxanthin and fucoxanthin induce apoptosis in PC-3 human prostate cancer cells. Cancer Lett. 2005;220(1):75-84.
14. Maeda H, Hosokawa M, Sashima T, Funayama K, Miyashita K. Effect of medium-chain triacylglycerols on anti-obesity effect of fucoxanthin. J Oleo Sci. 2007;56(12):615-21.
15. Sudhakar MP, Ananthalakshmi JS, Nair BB. Extraction, purification and study on antioxidant properties of fucoxanthin from brown seaweeds. J Chem Pharm Res. 2013;5(7):169-75.
16. Shannon E, Abu-Ghannam N. Optimisation of fucoxanthin extraction from Irish seaweeds by response surface methodology. J Appl Phycol. 2017;29(2):1027-36.
17. Li FL, Wang LJ, Fan Y, Parsons RL, Hu GR, Zhang PY. A rapid method for the determination of fucoxanthin in diatom. Mar Drugs. 2018;16(1):1-13.
18. Bezerra MA, Santelli RE, Oliveira EP, Villar LS, Escaleira LA. Response surface methodology (RSM) as a tool for optimization in analytical chemistry. Talanta. 2008;76(5):965-77.
19. Vera Candioti L, De Zan MM, Cámara MS, Goicoechea HC. Experimental design and multiple response optimization. Using the desirability function in analytical methods development. Talanta. 2014;124:123-38.
20. Billakanti JM, Catchpole OJ, Fenton TA, Mitchell KA, Mackenzie AD. Enzyme-assisted extraction of fucoxanthin and lipids containing polyunsaturated fatty acids from Undaria pinnatifida using dimethyl ether and ethanol. Process Biochem. 2013;48(12):1999–2008.
21. Yan X, Chuda Y, Suzuki M, Nagata T. Fucoxanthin as the Major Antioxidant in Hijikia fusiformis , a Common Edible Seaweed. Vol. 63, Bioscience, Biotechnology, and Biochemistry. 1999. p. 605-7.
22. Conde E, Moure A, Domínguez H. Supercritical CO2 extraction of fatty acids, phenolics and fucoxanthin from freeze-dried Sargassum muticum. J Appl Phycol. 2015;27(2):957-64.
23. Jang EJ, Kim SC, Lee JH, Lee JR, Kim IK, Baek SY, et al. Fucoxanthin, the constituent of Laminaria japonica, triggers AMPK-mediated cytoprotection and autophagy in hepatocytes under oxidative stress. BMC Complement Altern Med. 2018;18(1):1–11.
24. Raguraman V, Stanley Abraham L, Mubarak Ali D, Narendrakumar G, Thirugnanasambandam R, Kirubagaran R, et al. Unraveling rapid extraction of fucoxanthin from Padina tetrastromatica: Purification, characterization and biomedical application. Process Biochem. 2018;
25. Kim SM, Jung YJ, Kwon ON, Cha KH, Um BH, Chung D, et al. A potential commercial source of fucoxanthin extracted from the microalga Phaeodactylum tricornutum. Appl Biochem Biotechnol. 2012;166(7):1843–55.
26. Guo B, Liu B, Yang B, Sun P, Lu X, Liu J, et al. Screening of diatom strains and characterization of Cyclotella cryptica as a potential fucoxanthin producer. Mar Drugs. 2016;14(7).
27. Erdoğan A, Demirel Z, Conk Dalay M, Eroğlu AE. Fucoxanthin Content of Cylindrotheca closterium and Its Oxidative Stress Mediated Enhancement. Turkish J Fish Aquat Sci. 2016;16:499-506.
28. Machu L, Misurcova L, Ambrozova JV, Orsavova J, Mlcek J, Sochor J, et al. Phenolic content and antioxidant capacity in algal food products. Molecules. 2015;20(1):1118–-33.
29. Ozyurt VH, Otles S. Determination of 3-nitrotyrosine in food protein suspensions. Talanta. 2017.
30. Strati IF, Oreopoulou V. Effect of extraction parameters on the carotenoid recovery from tomato waste. Int J Food Sci Technol. 2011;46(1):23-9.
31. Samavati V. Polysaccharide extraction from Abelmoschus esculentus: Optimization by response surface methodology. Carbohydr Polym. 2013;95(1):588-97.
32. Mikami K, Hosokawa M. Biosynthetic pathway and health benefits of fucoxanthin, an algae-specific xanthophyll in brown seaweeds. Int J Mol Sci. 2013;14(7):13763-81.
33. Roh MK, Uddin MS, Chun BS. Extraction of fucoxanthin and polyphenol from Undaria pinnatifida using supercritical carbon dioxide with co-solvent. Biotechnol Bioprocess Eng. 2008;13(6):724-9.
34. Kanda H, Kamo Y, Machmudah S, Wahyudiono, Goto M. Extraction of fucoxanthin from raw macroalgae excluding drying and cell wall disruption by liquefied dimethyl ether. Mar Drugs. 2014;12(5):2383-96.
35. Sivagnanam SP, Yin S, Choi JH, Park YB, Woo HC, Chun BS. Biological properties of fucoxanthin in oil recovered from two brown seaweeds using supercritical CO2 extraction. Mar Drugs. 2015;13(6):3422–42.
36. Craft BD, Kerrihard AL, Amarowicz R, Pegg RB. Phenol-Based Antioxidants and the In Vitro Methods Used for Their Assessment. Compr Rev Food Sci Food Saf. 2012;11(2):148-73.
37. Manach C, Scalbert A, Morand C, Remesy C, Jimenez L. Polyphenols : food sources and bioavailability . Am J Clin Nutr. Am J Clin Nutr. 2004;79(October 2015):727-47.
38. Dang TT, Bowyer MC, Van Altena IA, Scarlett CJ. Comparison of chemical profile and antioxidant properties of the brown algae. Int J Food Sci Technol. 2017;174–81.
39. Chakraborty K, Praveen NK, Vijayan KK, Rao GS. Evaluation of phenolic contents and antioxidant activities of brown seaweeds belonging to Turbinaria spp. (Phaeophyta, Sargassaceae) collected from Gulf of Mannar. Asian Pac J Trop Biomed. 2013;3(1):816.
40. Yumiko YS, Ya-Pei H, Takeshi S. Distribution of flavonoids and related compounds from seaweeds in Japan. J Tokyo Univ Fish. 2003;89:16.